Web Directory

» A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications
   Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.
   http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb17/17053-1.pdf

» Adjuvants in PCR Reactions
   Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.
   http://info.med.yale.edu/genetics/ward/tavi/p16.html

» All about PCR
   Information about PCR such as PCR machines, PCR reagents and PCR news.
   http://www.pcrinfocentral.com

» Amberg Laboratory Protocols:
   Laboratory protocols for PCR work used by the group of David Amberg at the Department of Biochemistry and Molecular Biology Upstate Medical University, Syracuse, New York.
   http://www.upstate.edu/biochem/amberg/protocols.php

» Attotron Biosensor Corporation
   Research and development company for development of biosensors and related products for the research and educational markets.
   http://www.attotron.com

» BioRad, Amplification, PCR
   Division of BioRad Laboratories that manufactures and sells instruments for PCR, in Hercules, California, USA.
   http://www.bio-rad.com/B2B/BioRad/product/br_category.jsp?BV_SessionID=@@@@0937418475.1167690030@@@@&BV_EngineID=ccciaddjkighejmcfngcfkmdhkkdfll.0&divName=Life+Science+Research&loggedIn=false&serviceLevel=Lit+Request&lang=English&c

» DNALC: PCR Animation
   An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory, USA.
   http://www.dnalc.org/ddnalc/resources/shockwave/pcranwhole.html

» Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences
   Thesis abstract about restriction fragment length polymorphism (RFLP) by Alexander Binder 1997.
   http://www.uni-graz.at/~binder/thesis/node64.html

» Detection of Single Nucleotide Mutations in Wheat Using Single Strand Conformation Polymorphism Gels
   P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.
   http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb19/R01-013.pdf

» Dolan DNA Learning Centers Gene Almanac
   Educational site on topics in genetics and gene expression from Cold Spring Harbor Laboratory, USA.
   http://www.dnalc.org/home.html

» Effect of PCR Buffer on Multiplex PCR
   Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company).
   http://www.qiagen.com/literature/brochures/pcr/pdf/pcrcha03.pdf

» FISH Guide and Troubleshooting
   Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.
   http://info.med.yale.edu/genetics/ward/tavi/FISHguide.html

» Fidelity of DNA Polymerases for PCR
   Article by PN Hengen from TIBS 1995
   http://www.lecb.ncifcrf.gov/~pnh/papers/TIBS/aug95.html

» Fluorescence in Situ Hybridisation
   Technical notes on Fluorescence in situ hybridisation from the Institute of Genetics of Yale School of Medicine, New Haven, Connecticut, USA.
   http://info.med.yale.edu/genetics/ward/tavi/FISH.html

» GenHunter
   Manufacturer of material for differential display PCR in Nashville, Tenn USA.
   http://www.genhunter.com

» GeneOhm Sciences
   Tests on group B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing.
   http://www.geneohm.com

» HiFi DNA
   HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
   http://www.hifidna.com/

» Ingenetix GmbH
   Develops technology and products for DNA and mRNA research. Also provide DNA testing for the determination of parentage/paternity and custom DNA sequencing, oligonucleotide synthesis, genotyping services, pharmacogenetics and quantitative PCR, in Vienna,
   http://www.ingenetix.com/

» Inverse PCR Protocol
   Step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.
   http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/inverse_pcr_protocol.html

» Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation
   Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
   http://www.fruitfly.org/about/methods/inverse.pcr.html

» Inverse PCR for PAC-end Sequencing
   To generate PCR fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk, Washington University Zebrafish Genome Resources Project, USA.
   http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/prot2.html

» Inverse PCR for Use with Snyder mTn-lacZ/LEU2-based Mutagenesis
   Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.
   http://labs.fhcrc.org/gottschling/General%20Protocols/ipcr.html

» Kary B. Mullis - Autobiography
   The originator of PCR, from the Nobel e-museum web site.
   http://nobelprize.org/chemistry/laureates/1993/mullis-autobio.html

» Kary Mullis
   Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.
   http://www.invent.org/hall_of_fame/109.html

» Long PCR Protocol
   Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
   http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html

» Nematode ITS1 Size Variation
   Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes, from University of Nebrasca.
   http://nematode.unl.edu/its_id/EXAMPLES/index.htm

» Optimizing Multiplex and LA-PCR with Betaine
   LA-PCR = "long and accurate PCR". Article by PN Hengen in TIBS June 1997.
   http://www-lecb.ncifcrf.gov/~pnh/papers/TIBS/jun97.html

» Optimizing PCR Protocols
   Brief guidelines. From the Jackson Laboratory, University of Maine, USA.
   http://www.jax.org/imr/optimize_pcr.html

» PCR Amplification of cDNA Segments by 2 Stage Nested PCR
   Protocol from the method database of NIH, USA.
   http://www.ncbi.nlm.nih.gov/SNP/snp_viewTable.cgi?type=method&method_id=555

» PCR Animated
   Animation illustrating the principle of PCR, from the University of Ghent, Belgium.
   http://users.ugent.be/~avierstr/principles/pcrani.html

» PCR Gateway
   A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press.
   http://www.horizonpress.com/pcr/

» PCR Guru
   A downloadable textbook on PCR setup and optimization, not free.
   http://www.pcrguru.com/

» PCR Method Protocols
   Protocols for PCR posted by the Helen Donis-Keller Laboratory.
   http://hg.wustl.edu/hdk_lab_manual/pcr/pcrcontents.html

» PCR Project
   Presentations from the University of California at Berkley on PCR, both current research reports and reviews.
   http://sunsite.berkeley.edu/biotech/pcr/

» PCR Protocol
   Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.
   http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html

» PCR Protocols
   Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University
   http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html

» PCR Technology
   An introduction by Connie Veilleux from the US National Health Museum website.
   http://www.accessexcellence.org/LC/SS/PS/PCR/PCR_technology.html

» PCR Troubleshooting
   Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.
   http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html

» PCR World
   Collection of unreferenced texts on various aspects of PCR.
   http://pcrworld.blogspot.com

» PCR and Multiplex PCR Guide
   Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.
   http://www.info.med.yale.edu/genetics/ward/tavi/Guide.html

» PCR-ELISA and Related
   P Zhang, CJ Gebhart, D Burden, GE Duhamel: A low technology alternative to real time PCR, technical article on the site of BT&C, Inc Bridgewater, NJ, USA.
   http://www.btc-bti.com/pcrelisa.htm

» Polymerase Chain Reaction
   Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.
   http://www.accessexcellence.com/RC/CT/polymerase_chain_reaction.html

» Polymerase Chain Reaction (PCR)
   A graphic description of the principle of PCR from the US National Health Museum web site.
   http://www.accessexcellence.org/AB/GG/polymerase.html

» PrimerDigital
   International biotechnology company specialized in a design service for PCR primers and probes, PCR-based technology development, projects for development of polymorphism and software development.
   http://primerdigital.com/index.php

» Primerfox
   Free online tool for generation of PCR primers.
   http://www.primerfox.com

» Principle of PCR
   Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.
   http://users.ugent.be/~avierstr/principles/pcr.html

» Protocols Online: PCR Protocols
   Extensive collection of PCR protocols and methods from Protocol On Line.
   http://www.protocol-online.org/prot/Molecular_Biology/PCR/

» Quantitative PCR Protocol
   From the Jackson Laboratory, University of Maine, USA.
   http://www.jax.org/cyto/quanpcr.html

» RAPD PCR
   RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA.
   http://avery.rutgers.edu/WSSP/StudentScholars/project/archives/onions/rapd.html

» Rational Primer Design Greatly Improves Differential Display-PCR (DD-PCR)
   Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.
   http://nar.oxfordjournals.org/cgi/content/full/25/11/2239

» Reference in PCR
   Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.
   http://www.gene-quantification.org

» Rep-PCR Genomic Fingerprinting
   Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.
   http://www.msu.edu/user/debruijn/

» Roe Laboratory Protocols
   Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
   http://www.genome.ou.edu/proto.html

» Single Tube Confirmation PCR Protocol
   For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.
   http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

» T-DNA Generated Enhancer Traps in Arabidopsis
   Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.
   http://www.dartmouth.edu/~tjack/

» Tavi's PCR Protocols
   A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.
   http://info.med.yale.edu/genetics/ward/tavi/PCR.html

» The PCR Encyclopedia
   Describes plans for a free encyclopedia dedicated to the polymerase chain reaction (PCR).
   http://www.pcr-encyclopedia.com/

» The Web Guide of PCR
   List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.
   http://www.pcrlinks.com/

» Thermostable DNA Polymerases
   Discussion of their origin and briefly their properties. From the web site of Colorado State University.
   http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/hotpolys.html

» Wayward PCR Primers
   Article by PN Hengen from TIBS 1995 on the loss of activity of PCR primers with time.
   http://www-lecb.ncifcrf.gov/~pnh/papers/TIBS/jan95.html

» What the Heck is PCR?
   Popular description of the PCR technique by John C Brown, University of Kansas 1995.
   http://people.ku.edu/~jbrown/pcr.html

» Which DNA Marker for Which Purpose?
   Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Gött
   http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarker